All About Probes | In Situ Hybridization, RNA-ISH | ACDBio

来自 : 发布时间：2024-04-28

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PRODUCT AVAILABILITY: Update Regarding the Evolving COVID-19 Situation

Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally. Bio-Techne WHERE SCIENCE INTERSECTS INNOVATIONTM

Close All About ProbesAll About ProbesCatalog ProbesRNA Target Probes Made-to-OrderRNAscope® and Basescope™ Probes

The RNAscope® and BaseScope™ Assays are based on the proprietary RNAscope ® Technology enabling RNA target expression analysis within intact cells and tissues with high sensitivity and specificity. Learn more about the difference between RNAscope® and BaseScope™ Assays.

Catalog and Made-to-Order Probes

Using proprietary ACD RNAscope® Probe Design Pipeline, we provide double \"Z\" oligo probes designed to hybridize to your specific RNA target of interest. We can design in situ hybridization RNA probes for virtually ANY gene in ANY genome for visualization in ANY tissue or Cells.

Catalog Target Probes

Previously designed and used by our customers, they can be shipped to you in approx. 2 weeks. Our catalog probes span over 13,000 RNA targets in over 140 species including human, mouse, rat, dog, cow, zebrafish, rabbit, pig, chicken, monkeys and over 100 viruses. Search and select from our over ever-growing catalog of in situ hybridization probe sets.

RNAscope® Made-to-Order Target Probes

New probes for your gene of interest will be designed and manufactured in about 2 weeks.

Probe Exclusivity

Under certain conditions, ACD may agree to grant exclusivity to probes against \"proprietary and private\" targets for a fee. While ACD still owns the probe design for these targets, we are committed to not market these probes until the probe exclusivity is removed. Please talk to your account executive for further information.

Probe Name NomenclatureRNAscope® Probe Name NomenclatureProbe Probe TypeHs SpeciesFDXR Gene5UTR SpecificationC2 Amplification Channel

These are three types of RNAscope probes. The type of probe is indicated as follows:

Probefor Manual Assays

LS Probefor Leica Systems automated assays

VS Probefor Ventana Systems automated assays

Species refers to the probe target species and implies that the probe is designed to the gene of the specified species. The species is typically identified by two letter designations such as \"Hs\" for Homo Sapiens, \"Mm\" for Mus musculus etc

This refers to the Official Gene Symbol as listed in the NCBI Database

This indicates any specific target region within the gene

This indicates the probe amplification channel denoted by C1, C2 or C3. RNAscope probes default to Channel 1 unless specified. This channel indicates the compatibility of the probes with different amplification systems and indicate which detection kits the probes would be compatible with.

C1 probes are compatible with all detection platforms

C2 probes are compatible only with the 2.5 HD Duplex Detection Kit and the Fluorescent Detection Kit

C3 channel probes are only compatible with the Fluorescent Detection Kit

Example 1LS Probe - Mm-Rspo3

This probe used in Leica Automated System Assay(LS Probe) and is designed to hybridize to Mus musculus (Mm) Rspo3 gene. Its spectral channel is not listed which means Spectral Channel 1 and therefore compatible with any of the four manual asays including RNAscope® 2.5 HD Reagent Kit-BROWN, RNAscope® 2.5 HD Reagent Kit-RED, RNAscope® 2.5 HD Duplex Reagent Kit or RNAscope® Multiplex Fluorescent Reagent Kit.

Example 2Probe-Hs-GNRHR-5UTR-C2

This is probe is designed to be used with a manual assay (Probe). It targets just the 5\' untranslated region (5UTR) of the GNRHR gene from the human sequence(Hs). This probe is compatible with spectral channel 2 (C2), so it can be used in a duplex or multiplex assay.

 

BaseScope™ Probe Name NomenclatureBA PlatformMm SpeciesNrg1 GeneE1E2 Sub-Type

The BaseScope™ Assay is a recent addition to the RNAscope® Technology product portfolio, launched in early 2016. Using the same innovative technology and proprietary probe design as used in the RNAscope Assay, the BaseScope Assay is further refined to detect as few as 1-4 Z pairs.

Species refers to the probe target species and implies that the probe is designed to the gene of the specified species. The species is typically identified by two letter designations such as \"Hs\" for Homo Sapiens, \"Mm\" for Mus musculus etc

This refers to the Official Gene Symbol as listed in the NCBI Database

BaseScope technology enables the specific detection of mutations, exon junctions, short target sequences, highly homologous sequences, circular RNA and fusion genes with single molecule detection sensitivity in a broad range of tissues, samples and species. These sub-type helps classify probes for the intended applications.

As an example in this probe E1E2 designates that this probe spans across junction of Exon 1 and 2 of the NRG1 Gene

ExampleBaseScope™ Probe - BA-Dm-Gapdh1-3zz

This probe is compatible with Manual BaseScope™ assays and is designed to hybridize to Drosophila melanogaster Gapdh1 gene. The 3ZZ specifies the number of probe pairs and indicates this is a short target probe.

Probe Compatibility

ProbesAssay Compatibility

RNAscope® Manual Assays

RNAscope® 2.5 LS (Leica Biosystems)

RNAscope® 2.5 VS (Ventana Systems)

BaseScope™ Manual Assay

Probe Name Naming convention

Diaminobenzidine(DAB)RNAscope ProbeEg: Probe - Hs-FDXR-C2

2.5 LS RNAscope® ProbeEg: 2.5 LS Probe - Hs-FDXR

2.5 VS RNAscope® ProbeEg: 2.5 VS Probe - Hs-FDXR

BaseScope™ ProbeEg: BA-Mm-Hdgfrp3-E3E4

Probe naming convention

Probe Type-Species-GeneName-Channel

Probe Type-Species-GeneName

Probe Type-Species-GeneName

Platform-Species-Gene-Sub-Type

Spectral Channel Compatibility

Available for Channel 1-4(C1, C2,C3, C4)When Channel No. is missing, the probe is a C1 probe

Available for Channel 1-4(C1, C2,C3, C4)When Channel No. is missing, the probe is a C1 probe

Available for Channel 1 and 2 Only.When Channel No. is missing, the probe is a C1 probe.

Channel 1 and 2 Only. When Channel No. is missing, the probe is a C1 probe.

C1 Probes Assay Compatibility

C1 is spectral channel 1 and is the most abundant probe type.RNAscope® 2.5 HD Reagent Kit--BROWN

RNAscope® 2.5 HD Reagent Kit--RED

RNAscope® 2.5 HD Duplex Reagent Kit

RNAscope® Fluorescent Multiplex Reagent Kit

RNAscope® Multiplex Fluorescent Reagent Kit v2

RNAscope® 2.5 LS Assay Reagent Kit--BROWN

RNAscope® 2.5 LS Assay Reagent Kit--RED

RNAscope® 2.5 LS Duplex Reagent Kit

RNAscope® LS Multiplex Fluorescent Reagent Kit

RNAscope® VS Universal HRP Reagent Kit

RNAscope® VS Universal AP Reagent Kit

RNAscope® VS Duplex Reagent Kit

BaseScope™ Reagent Kit v2–RED

BaseScope™ Duplex Reagent Kit

C2 Probes Assay Compatibility

C2 is spectral channel 2RNAscope® 2.5 HD Duplex Reagent Kit

RNAscope® Fluorescent Multiplex Reagent Kit

RNAscope® Multiplex Fluorescent Reagent Kit v2

RNAscope® 2.5 LS Duplex Reagent Kit

RNAscope® LS Multiplex Fluorescent Reagent Kit

RNAscope® VS Duplex Reagent Kit

BaseScope™ Duplex Reagent Kit

C3 Probes Assay Compatibility

C3 is spectral channel 3RNAscope® Fluorescent Multiplex Reagent Kit

RNAscope® Multiplex Fluorescent Reagent Kit v2

RNAscope® LS Multiplex Fluorescent Reagent Kit

Not Available

Not Available

C4 Probes Assay Compatibility

C4 is spectral channel 4RNAscope® 4-Plex Ancillary Kit for Multiplex Fluorescent Kit v2

RNAscope® LS 4-Plex Ancillary Kit for LS Multiplex Fluorescent

Not Available

Not Available

Probe Volume

Probes for 20 slides

Probes for 30 slides

Probes for 30 slides

Probes for 20 slides

Species Designation Probe name contains two letters to indicate species name

Hs-Homo SapienMm-Mus musculusRt-Rattus norvegicusACD offers 40 species

Hs-Homo SapienMm-Mus musculusRt-Rattus norvegicusACD offers 40 species

Hs-Homo SapienMm-Mus musculusRt-Rattus norvegicusACD offers 40 species

Hs-Homo SapienMm-Mus musculusRt-Rattus norvegicusACD offers 40 species

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